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WorkBeads™ 40 ACT and WorkBeads 40/10000 ACT "NEW"

Activated Media for Preparative and Bioprocess Scale Affinity Chromatography with User's Choice of Ligands

    .  Made from Agarose, Well Established and Well-known in the Biotechnology Industry
    .  Simple Coupling Procedures at Room Temperature
    .  Stable at Room Temperature in Aqueous Solution and at Neutral pH
    .  Suitable for Coupling of Ligands Containing Sulphydryl, Amino or Hydroxyl Groups

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT pre-activated separation media are produced from agarose using a proprietary cross linking method that result in a highly porous and physi-cally stable agarose matrix. Agarose based matrices have been successfully used for decades in bio-technology research and in the industrial purifica-tion of proteins. Agarose is proven to be exception-ally compatible with natural bio-molecules such as proteins, DNA, carbohydrates etc. The material shows minimal non specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the micro-environment in the column which lead to distorted separations.

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are activated according to the Bromohydrin method. This activation method is proprietary and based upon well known chemistry and allows you to perform the coupling chemistry in aqueous solu-tions.

Matrix-OCH2CH(OH)CH2Br + Nucleophil (i.e. -SH, -NH2 or -OH) --> Matrix-OCH2CH(OH)CH2-Nu


WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are supplied as an aqueous suspension with 22% ethanol as preservative. After washing, the gel is immediately ready for use. As no toxic chemicals are involved and the WorkBeads 40 ACT products are stable at room temperature the coupling proce-dure can, as long as your application so allows, easily be performed on your bench and at room temperature.

Applications

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are ready for use.

Proteins or other molecules with free amino and sulfhydryl l groups will couple easily to. Just add the ligand to the suspension, stir and incubate over night.
Due to the very high porosity WorkBeadsTM40/10 000 ACT is ideal for coupling large entities like immunglobulins. The exclusion limit is more than 10000 kD.
Hyd roxy groups can also be used for coupling but will require pH above 12 which is not compatible with most proteins. However, stable molecules could be coupled using the hydroxyl group. Re-maining reactive groups are deactivated using mercapto-ethanol or ethanol-amine

Order Information for WorkBeads 40 ACT and WorkBeads 40/10000 ACT