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WorkBeads™40 DEAE

Ion Exchange Media for Preparative and Bioprocess Scale Purification of Proteins

• Made from agarose, well established and well-known in the biotech industry
• High throughput and resolution
• Reliable and reproducible
• High chemical stability for easy cleaning in place
• Easy and reliable scale up

Media Description


WorkBeads40 DEAE ion exchange media are produced from agarose using a proprietary cross linking method that results in a highly porous and physically stable agarose matrix. Agarose based matrices have been successfully used for decades in biotechnology research and in the industrial purifi-cation of proteins. Agarose is proven to be excep-tionally compatible with natural bio molecules like proteins, DNA, carbohydrates etc. The material shows minimal non specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the microenvironment in the column which lead to distorted separations.

WorkBeads 40 DEAE have a high selectivity which means the protein peaks are well separated with greater distance from each other than compa-rable products made from synthetic polymers. This means that the media has capacity to separate pro-teins well even when using high proteins loadings.
DEAE is a weak ion exchanger meaning that above pH 9 and higher there will be very few ion ex-change groups facilitating CIP. The charge density is also lower that WorkBeads 40 Q which will give a different selectivity.

Resolution is the combined effect of selectivity (dis-tance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent se-lectivity. The particle size of 40 micrometer with an very narrow particle size distribution in combina-tion with the proprietary cross linking results in columns packed with very high efficiency and ex-
cellent flow characteristics well suited to demand-ing bioprocess applications.

Applications

WorkBeads 40 DEAE ion exchange media are de-signed for high throughput protein separations under a variety of conditions. The high resolution that can be obtained at high protein loadings and high flow rates makes it ideal for process applica-tions. The chemical stability means it is easy to de-velop cleaning in place (CIP) protocols using so-dium hydroxide.

Ordering Information