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WorkBeads™ 40 SEC, WorkBeads 40/100 SEC "NEW" and
WorkBeads 40/10000 SEC "NEW"

High Performance Size Exclusion Chromatography Media for Preparative and Process Scale Separation of Proteins

    .  Made From Agarose, Well Established and Well-known in the Biotech Industry
    .  Excellent Resolution
    .  Robust Separation Results Can be Achieved Across a Wide Range of Proteins & Conditions
    .  Chemically Stable for Cleaning in Place

Media Description

All WorkBeads™40 SEC media are produced from agarose using a proprietary cross linking method that results in a highly porous and physically stable agarose matrix. Agarose based matrices has been successfully used over decades in biotechnology research and in the industrial purification of pro-teins. Agarose is proven to be excellently compati-ble with natural bio-molecules like proteins, DNA carbohydrates etc. The material shows minimal non specific interaction due to hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the micro-environment in the column and distorted separa-tions

WorkBeads 40 SEC for Size Exclusion Chromatog-raphy or Gel Filtration Media has a high selectivity which means the protein peaks are well separated with greater distance from each other than compa-rable products made from synthetic polymers. This means that the media has capacity to separate pro-teins well even when using high proteins loadings.

Figure 1. shows the Kav curve for some common proteins. The dimer of thyroglobulin elutes in Vo and the other proteins nicely follow the theoretical Kav  relation.



Resolution is the combined effect of selectivity (dis-tance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent se-lectivity. The narrow particle size distribution around 40 micrometer in combination with the proprietary cross linking results a media that is easy to pack in columns with very high efficiency and good flow characteristics.

WorkBeads 40 SEC Gel Filtration Media is de-signed for high performance protein separations under a variety of conditions. The high resolution that can be obtained makes ideal for both prepara-tive work and process scale separation of proteins.

Figure 1. shows the Kav curve for some common proteins. The dimer of thyroglobulin elutes in Vo and the other proteins nicely follow the theoretical Kav curve.

Resolution is the combined effect of selectivity (distance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent selectivity. The narrow particle size distribution around 40 micrometer in combination with the proprietary cross linking results a media that is easy to pack in columns with very high efficiency and good flow characteristics.

Separation Ranges

Ordering Information

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